- Genomic/metabolomic/proteomic approaches for identification of novel (regulatory and biosynthetic) aroma genes
- Metabolic engineering of plants and yeast
- Site-specific genome modification and genetic engineering in plants
Funding: Ministry of Agriculture; Ministry of Industry, Trade and Labor; Ministry of Science; The Israel Science Foundation; United States-Israel Binational Agricultural Research and Development Fund (BARD); German-Israeli Foundation (GIF); AID-CDR; Yissum (private companies).
- MemoGene: Site Specific Genome Modification (Link)
- MemoGene & Gene-validation services: Yissum Technolgy Transfer (PDF)
- Enhancing The Fragrance of Flowers (Video - CNN)
- Face of Israel: Professor Vainstein (Video - Israel21c)
- Plant Scent Enhancement (Video - Yissum - Israel21c)
- Israeli Team Produces Anti-Malaria Drug From Tobacco Plants (Wall Street Daily) (Link)
- Anti-Malaria Drug Produced from Tobacco Plants (Link & Video - CCTV news)
- Tobacco could make Anti-Malaria Drug CCTV News (Video - CNTV English)
- The News Today with Ayman Sikseck
- Découvrez la fleur génétiquement modifiée fierté d'Israël
Two showy traits, scent emission and pigmentation, are finely coregulated by the MYB transcription factor PH4 in petunia flowers
The mechanism underlying the emission of phenylpropanoid volatiles is poorly understood. Here, we reveal the involvement of PH4, a petunia MYB-R2R3 transcription factor previously studied for its role in vacuolar acidification, in floral volatile emission. We used the virus-induced gene silencing (VIGS) approach to knock down PH4 expression in petunia, measured volatile emission and internal pool sizes by GC-MS, and analyzed transcript abundances of scent-related phenylpropanoid genes in flowers. Silencing of PH4 resulted in a marked decrease in floral phenylpropanoid volatile emission, with a concurrent increase in internal pool levels. Expression of scent-related phenylpropanoid genes was not affected. To identify putative scent-related targets of PH4, we silenced PH5, a tonoplast-localized H+-ATPase that maintains vacuolar pH homeostasis. Suppression of PH5 did not yield the reduced-emission phenotype, suggesting that PH4 does not operate in the context of floral scent through regulation of vacuolar pH. We conclude that PH4 is a key floral regulator that integrates volatile production and emission processes and interconnects two essential floral traits -olor and scent. (New Phytologist (2015) 208: 708-4)
Petunia floral scent production is negatively affected by high-temperature growth conditions
Increasing temperatures due to changing global climate are interfering with plant-llinator mutualism, an interaction facilitated mainly by floral colour and scent. Gas chromatography-mass spectroscopy analyses revealed that increasing ambient temperature leads to a decrease in phenylpropanoid-based floral scent production in two Petunia xybrida varieties, P720 and Blue Spark, acclimated at 22/16 or 28/22 °C (day/night). This decrease could be attributed to down-regulation of scent-related structural gene expression from both phenylpropanoid and shikimate pathways, and up-regulation of a negative regulator of scent production, emission of benzenoids V (EOBV). To test whether the negative effect of increased temperature on scent production can be reduced in flowers with enhanced metabolic flow in the phenylpropanoid pathway, we analysed floral volatile production by transgenic 'Blue Spark' plants overexpressing CaMV 35S-driven Arabidopsis thaliana production of anthocyanin pigments 1 (PAP1) under elevated versus standard temperature conditions. Flowers of 35S:PAP1 transgenic plants produced the same or even higher levels of volatiles when exposed to a long-term high temperature regime. This phenotype was also evident when analysing relevant gene expression as inferred from sequencing the transcriptome of 35S:PAP1 transgenic flowers under the two temperature regimes. Thus, up-regulation of transcription might negate the adverse effects of temperature on scent production. (Plant, Cell and Environment (2015) 38, 1333-46)
Towards Tailor-Made Crops And Compounds
Many attempts have been made to convert plants into efficient factories. The ability to modify genome sequences in plant cells is fundamental to modern agriculture, but current methods - classical and moleculular - are extremely inefficient. To overcome the biotechnological barriers to precise genome editing, we developed MemoGene technology for the creation of commercially successful cultivars in leading high-value plants. MemoGene (commercially developed by Danziger Innovations Ltd.) is a viral-based tissue-culture-independent technology that bypasses traditional genetic engineering for precise plant-genome modification in all plants. It is based on highly efficient viral vectors for DNA delivery and targeted endonucleases for nuclear and plastid genome manipulations: site-specific genetic modifications can be applied to plants and cell cultures, to meet the rapidly shifting trends in the areas of field and vegetable crops, horticulture, woody crops, bio-fuels, etc.
Secondary metabolites determine color, flavor, fragrance and the health-beneficial nutritional/pharmacological value of foods, beverages, detergents, cosmetics and pharmaceutical products. Tools allowing efficient metabolic engineering of these products have a major impact on the almost limitless world bio-agriculture market. Our characterization of novel plant-regulatory factors allowed us to genetically engineer petunia flowers with ca. 10-fold higher scent production/emission, and to transform naturally white flowering gypsophila, sold worldwide, into a novel cut-flower crop with purple flowers (currently being commercially developed by Danziger "Dan" Flower Farm). Our studs in yeast of factors regulating production of the secondary metabolite artemisinin, the most important antimalarial drug today, aim to boost artemisinin production in host Artemisia annua plants, and to produce it in tobacco and aspen. The drug will be produced at high levels in planta by inducing the expression of five genes necessary for its synthesis in specific tissues, cell types and intracellular compartments at specific developmental stages. The development of yeast/plant-based approaches (supported by Isaac Kaye award) for this drug's cost-effective production will bring this much awaited remedy to developing nations.
PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers
Floral scent is a complex trait of biological and applied significance. To evaluate whether scent production originating from diverse metabolic pathways (e.g. phenylpropanoids and isoprenoids) can be affected by transcriptional regulators, Arabidopsis PAP1 transcription factor was introduced into Rosa hybrida.In addition to increased levels of phenylpropanoid-derived color and scent compounds as compared to control flowers, PAP1-transgenic rose lines also emitted up to 6.5 times higher levels of terpenoid scent compounds. Olfactory assay revealed that bees and humans could discriminate between the floral scents of PAP1-transgenic and control flowers. The increase in volatile production in PAP1 transgenes was not due solely to transcriptional activation of their respective biosynthetic genes but probably also resulted from enhanced metabolic flux in both the phenylpropanoid and isoprenoid pathways. (New Phytologist 195:xx ( 2012)
Generation Of The Potent Anti-Malarial Drug Artemisinin In Tobacco
The emergence of multidrug-resistant strains of Plasmodium spp., the etiological agent of malaria, constitutes a major threat to controlling the disease. Artemisinin, a natural compound from Artemisia annua (sweet wormwood) plants, is highly effective against drug-resistant malaria. The World Health Organisation (WHO; Geneva) promotes the use of artemisinin as a first-line treatment for malaria, and it is heavily involved in facilitating the development of artemisinin-based anti-malaria drugs1.Even so, low-cost artemisinin-based drugs are lacking because of the high cost of obtaining natural or chemically synthesized artemisinin. Despite extensive effort invested in the past decade in metabolic engineering of artemisinin and its precursors in both microbial and heterologous plant systems, production of artemisinin itself has never been achieved. Here we report the metabolic engineering of tobacco to produce artemisinin, generating transgenic plants that express five plant- and yeast-derived genes involved in the mevalonate and artemisinin pathways, all expressed from a single vector. Our experiments demonstrate that artemisinin can be fully biosynthesized in a heterologous (that is, other than A. annua) plant system, such as tobacco. The developed experimental platform should lead to the design of new routes for the drug’s commercial production in heterologous plant systems. (Nature Biotechnology 29:1072 (2012)
Harnessing yeast subcellular compartments for the production of plant terpenoids
The biologically and commercially important terpenoids are a large and diverse class of natural products that are targets of metabolic engineering. However, in the context of metabolic engineering, the otherwise well-documented spatial subcellular arrangement of metabolic enzyme complexes has been largely overlooked. To boost production of plant sesquiterpenes in yeast, we enhanced flux in the mevalonic acid pathway toward farnesyl diphosphate (FDP) accumulation, and evaluated the possibility of harnessing the mitochondria as an alternative to the cytosol for metabolic engineering. Overall, we achieved 8- and 20-fold improvement in the production of valencene and amorphadiene, respectively, in yeast co-engineered with a truncated and deregulated HMG1, mitochondrion-targeted heterologous FDP synthase and a mitochondrion-targeted sesquiterpene synthase, i.e. valencene or amorphadiene synthase. The prospect of harnessing different subcellular compartments opens new and intriguing possibilities for the metabolic engineering of pathways leading to valuable natural compounds. Metabolic Eng. 13:474 (2011)
Nontransgenic Genome Modification in Plant Cells
Zinc finger nucleases (ZFNs) are a powerful tool for genome editing in eukaryotic cells. ZFNs have been used for targeted mutagenesis in model and crop species. In animal and human cells, transient ZFN expression is often achieved by direct gene transfer into the target cells. Stable transformation, however, is the preferred method for gene expression in plant species, and ZFN-expressing transgenic plants have been used for recovery of mutants that are likely to be classified as transgenic due to the use of direct gene-transfer methods into the target cells. Here we present an alternative, nontransgenic approach for ZFN delivery and production of mutant plants using a novel Tobacco rattle virus (TRV)-based expression system for indirect transient delivery of ZFNs into a variety of tissues and cells of intact plants. TRV systemically infected its hosts and virus ZFN-mediated targeted mutagenesis could be clearly observed in newly developed infected tissues as measured by activation of a mutated reporter transgene in tobacco (Nicotiana tabacum) and petunia (Petunia hybrida) plants. The ability of TRV to move to developing buds and regenerating tissues enabled recovery of mutated tobacco and petunia plants. Sequence analysis and transmission of the mutations to the next generation confirmed the stability of the ZFN-induced genetic changes. Because TRV is an RNA virus that can infect a wide range of plant species, it provides a viable alternative to the production of ZFN-mediated mutants while avoiding the use of direct plant-transformation methods. Trends in Biotech. 26:363 (2011)
EOBII, a Gene Encoding a Flower-Specific Regulator of Phenylpropanoid Volatiles' Biosynthesis in Petunia
Floral scent, which is determined by a complex mixture of low molecular weight volatile molecules, plays a major role in the plantג€™s life cycle. Phenylpropanoid volatiles are the main determinants of floral scent in petunia (Petunia hybrida). A screen using virus-induced gene silencing for regulators of scent production in petunia flowers yielded a novel R2R3-MYB-like regulatory factor of phenylpropanoid volatile biosynthesis, EMISSION OF BENZENOIDS II (EOBII). This factor was localized to the nucleus and its expression was found to be flower specific and temporally and spatially associated with scent production/emission. Suppression ofEOBII expression led to significant reduction in the levels of volatiles accumulating in and emitted by flowers, such as benzaldehyde, phenylethyl alcohol, benzylbenzoate, and isoeugenol. Up/downregulation of EOBII affected transcript levels of several biosynthetic floral scent-related genes encoding enzymes from the phenylpropanoid pathway that are directly involved in the production of these volatiles and enzymes from the shikimate pathway that determine substrate availability. Due to its coordinated wide-ranging effect on the production of floral volatiles, and its lack of effect on anthocyanin production, a central regulatory role is proposed for EOBII in the biosynthesis of phenylpropanoid volatiles. Plant Cell 22:1961 (2010)
Navigating the network of floral scent production
Flower fragrance is a composite character determined by secondary metabolites of diverse biosynthetic origin. Together with other traits, such as flower color, it is used by plants to lure pollinators and seed dispersers, thus ensuring plant survival. Research into the regulatory mechanisms leading to floral scent production/emission is still in its infancy and even less is known regarding flow within and cross-talk between secondary metabolic pathways leading to floral scent production. Using transgenic plants modified in anthocyanin production, we revealed an intriguing interrelationship between the branches of the phenylpropanoid pathway leading to the production of anthocyanins and volatiles. Specifically, we recorded five- to sevenfold higher levels of the volatile phenylpropanoids methyl benzoate and 2-hydroxymethyl benzoate in flavanone 3-hydroxylase (F3h)-suppressed carnation flowers with dramatically reduced anthocyanin levels, as compared to control non-transgenic flowers. Furthermore, overexpression in petunia flowers of the transcriptional regulator Pap1 (production of anthocyanin pigment 1), which activates the phenylpropanoid pathway, led to increases in both anthocyanin accumulation and volatile phenylpropanoid emission. Using virus-induced gene silencing (VIGS) for large-scale identification of floral scent genes, we further characterized metabolic flow within the pathway. The advantages of VIGS and of petunia as a model plant create a solid infrastructure for the future isolation of regulatory factors involved in floral scent production/emission. Knowledge gained from an understanding of mechanisms leading to floral scent production/emission should provide us with better insight into nature's way of ensuring evolutionary success, as well as with advanced tools for the metabolic engineering of fragrance. Plant Physiol. 145:1241 (2007); Plant Biotech. Journal 8:403 (2008)
Generation of phenylpropanoid pathway-derived volatiles in transgenic plants: rose alcohol acetyltransferase produces phenylethyl acetate and benzyl acetate in petunia flowers
Esters are important contributors to the aroma of numerous flowers and fruits. Acetate esters such as geranyl acetate, phenylethyl acetate and benzyl acetate are generated as a result of the action of alcohol acetyltransferases (AATs). Numerous homologous AATs from various plants have been characterized using in-vitro assays. To study the function of rose alcohol acetyltransferase (RhAAT) in planta, we generated transgenic petunia plants expressing the rose gene under the control of a CaMV-35S promoter. Although the preferred substrate of RhAAT in vitro is geraniol, in transgenic petunia flowers, it used phenylethyl alcohol and benzyl alcohol to produce the corresponding acetate esters, not generated by control flowers. The level of benzyl alcohol emitted by the flowers of different transgenic lines was ca. three times higher than that of phenylethyl alcohol, which corresponded to the ratio between the respective products, i.e. ca. three times more benzyl acetate than phenylethyl acetate. Feeding of transgenic petunia tissues with geraniol or octanol led to the production of their respective acetates, suggesting the dependence of volatile production on substrate availability. Plant Mol. Biol. 72:235 (2010)
Flower proteome: changes in protein spectrum during the advanced stages of rose petal development
Flowering is a unique and highly programmed process, but hardly anything is known about the developmentally regulated proteome changes in petals. Here, we employed proteomic technologies to study petal development in rose (Rosa hybrida). Using two-dimensional polyacrylamide gel electrophoresis, we generated stage-specific (closed bud, mature flower and flower at anthesis) petal-protein maps with ca. 1,000 unique protein spots. Expression analyses of all resolved protein spots revealed that almost 30% of them were stage-specific, with ca. 90 protein spots for each stage. Most of the proteins exhibited differential expression during petal development, whereas only ca. 6% were constitutively expressed. Eighty-two of the resolved proteins were identified by mass spectrometry and annotated. Classification of the annotated proteins into functional groups revealed energy, cell rescue, unknown function (including novel sequences) and metabolism to be the largest classes, together comprising ca. 90% of all identified proteins. Interestingly, a large number of stress-related proteins were identified in developing petals. Analyses of the expression patterns of annotated proteins and their corresponding RNAs confirmed the importance of proteome characterization. Planta 222:37 (2005)
Expression and functional analyses of the plastid lipid-associated protein CHRC suggest its role in chromoplastogenesis and stress
Chromoplastogenesis during flower development and fruit ripening involves the dramatic overaccumulation of carotenoids sequestered into structures containing lipids and proteins, termed PAPs (plastid lipid-associated proteins). CHRC, a cucumber (Cucumis sativus) PAP, has been suggested to be transcriptionally activated in carotenoid-accumulating flowers by gibberellic acid (GA). Mybys, a MYB-like trans-activator identified in this study, may represent a chromoplastogenesis-related factor: its expression is flower-specific and parallels that of ChrC during flower development; moreover, as revealed by stable ectopic and transient-expression assays, it specifically trans-activates ChrC promoter in flowers accumulating carotenoids and flavonoids. A detailed dissection of ChrC promoter revealed a GA-responsive element, gacCTCcaa, the mutation of which abolished ChrC activation by GA. This cis-element is different from the GARE motif and is involved in ChrC activation, probably via negative regulation, similar to other GA-responsive systems. The GA responsiveness and MYBYS floral activation of the ChrC promoter do not overlap with respect to cis-elements. To study the functionality of CHRC, which is activated in vegetative tissuessimilar to other PAPsby various biotic and abiotic stresses, we employed a tomato plant system and generated RNAi-transgenic lines with suppressed LeCHRC. Transgenic flowers accumulated ca. 30% less carotenoids per unit protein than controls, indicating an interrelationship between PAPs and flower-specific carotenoid accumulation in chromoplasts. Moreover, the transgenic LeCHRC-suppressed plants were significantly more susceptible to Botrytis cinerea infection, suggesting CHRC's involvement in plant protection under stress conditions and supporting the general, evolutionarily preserved role of PAPs. Plant Physiol. 142:233 (2008). Plant Physiol. 104:321 (1994).
CHRD, a plant member of the evolutionarily conserved YjgF family, influences photosynthesis and chromoplastogenesis
As noted above, studies on the carotenoid-overaccumulating structures in chromoplasts led to the characterization of PAPs, involved in the sequestration of hydrophobic compounds. Here we characterized the PAP CHRD, which, based on sequence homology, belongs to a highly conserved group of proteins, YER057c/YjgF/UK114, involved in the regulation of basic and vital cellular processes in bacteria, yeast and animals. Two nuclear genes were characterized in tomato plants: one (LeChrDc) is constitutively expressed in various tissues and the other (LeChrDi) is induced by stress in leaves and is upregulated by developmental cues in floral tissues. Using RNAi and antisense approaches, we showed their involvement in biologically significant processes such as photosynthesis. The quantum yield of photosynthetic electron flow in transgenic tomato leaves with suppressed LeChrDi/c expression was 30 to 50% of that in control, non-transgenic counterparts and was ascribed to lower PSI activity. Transgenic flowers with suppressed LeChrDi/c also accumulated up to 30% less carotenoids per unit protein as compared to control plants, indicating an interrelationship between PAPs and floral-specific carotenoid accumulation in chromoplasts. We suggest that CHRD's role in the angiosperm reproductive unit may be a rather recent evolutionary development; its original function may have been to protect the plant under stress conditions by preserving plastid functionality. Planta 225:89 (2006)
Synthesis of the food flavoring methyl benzoate by genetically engineered Saccharomyces cerevisiae
Current means of production for plant-derived aroma compounds include chemical synthesis and extraction from plant material. Both methods are environmentally detrimental and relatively expensive: plant material is only seasonally available and only a small subset of the plant biomass produces the desired aroma compounds, while organic synthesis inevitably involves waste byproducts with a negative ecological impact. Benzenoids are a class of plant metabolites that includes a number of aroma compounds. This research explores, for the first time, the feasibility of producing benzenoids in yeast. We elucidated a method for the production of the phenylpropanoid methyl benzoate in Saccharomyces cerevisiae using benzoic acid as the substrate, via heterologous expression ofAntirrhinum majus benzoic acid methyl transferase. Production was pH-dependent with a maximal yield of approximately 50 micrograms of methyl benzoate per liter of culture per hour, and with linear kinetics for at least 24 h. In addition, we analyzed two alternative expression vectors for the production of benzoic acid methyl transferase in S. cerevisiae: a constitutive triosephosphate isomerase promoter-based system was compared with a copper-inducible CUP1 promoter system. We found major differences in the amounts of methyl benzoate produced by these respective systems. Journal of Biotech. 122:307 (2006)
- Firsov, A., Shaloiko, L., Kozlov, O., Vinokurov, L., Vainstein, A. and Dolgov, S. (2016). Purification and characterization of recombinant supersweet protein thaumatin II from tomato fruit. Protein Expression and Purification. doi: 10.1016/j.pep.2016.03.002.
- Tzin, V., Rogachev, I., Meir, S., Moyal Ben Zvi, M., Masci, T., Vainstein, A., Aharoni, A. and Galili, G. (2015) Altered levels of aroma and volatiles by metabolic engineering of shikimate pathway genes in tomato fruits. AIMS Bioengineering 2: 75-92.
- Cna'ani, A., Spitzer-Rimon, B., Ravid, J., Farhi, M., Masci, T., Aravena-Calvo, J., Ovadis, M. and Vainstein, A. (2015) Two showy traits, scent emission and pigmentation, are finely co-regulated by the MYB transcription factor PH4 in petunia flowers. New Phytologist 208: 708-714 (cover).
- Honig, A., Marton, I., Rosenthal, M., Smith J.J., Nicholson, M.G., Jantz, D., Zuker, A. and Vainstein A. (2015) Transient expression of virally delivered meganuclease in planta generates inherited genomic deletions Mol. Plant 8: 1292-1294.
- Firsov, A., Tarasenko, I., Mitiouchkina, T., Ismailova, N., Shaloiko, L., Vainstein, A. and Dolgov, S. (2015) High-yield expression of M2e peptide of avian influenza virus H5N1 in transgenic duckweed plants. Mol. Biotechnol. 57: 653-661.
- Peer, R., Rivlin, G., Golobovitch, S., Lapidot, M., Gal-On, A., Vainstein, A., Tzfira, T., Flaishman, M.A. (2015) Targeted mutagenesis using zinc-finger nucleases in perennial fruit trees. Planta 241: 941-951.
- Cna'ani, A., Mühlemann, J.K., Ravid, J., Masci, T., Klempien, A., Nguyen, T.T.H., Dudareva, N., Pichersky, E. and Vainstein, A. (2015) Petunia x hybrida floral scent production is negatively affected by high-temperature growth conditions. Plant, Cell and Environ. 38: 1333-1346.
- Tzin, V., Rogachev, I., Meir, S., Moyal, Moyal Ben Zvi, M., Masci, T., Vainstein, A., Aharoni, A. and Galili, G. (2013) Tomato fruits expressing a bacterial feedback-insensitive 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase of the shikimate pathway possess enhanced levels of multiple specialized metabolites and upgraded aroma. J. Exp. Botany 64: 4441-4452
- Alon, M., Malka, O., Eakteiman, G., Elbaz, M., Moyal Ben Zvi, M., Vainstein, A. and Morin, S. (2013) Activation of the phenylpropanoid pathway in Nicotiana tabacum improves the performance of the whitefly Bemisia tabaci via reduced jasmonate signaling. Plos One 8 (10): e76619. doi:10.1371/journal.pone.0076619
- Marton, I., Honig, A., Omid, A., De Costa, N., Marhevka, M., Cohen, B., Zuker, A. and Vainstein, A. (2013) From Agrobacterium to viral vectors: genome modification of plant cells by rare cutting restriction enzymes. Int. J. Dev. Biol. 57: 639-650.
- Barberini, S., Savona, M., Raffi, D., Leonardi, M., Pistelli, La., Stochmal, A., Vainstein, A, Pistelli, Lu. and Ruffoni B. (2013) Molecular cloning of SoHPPR encoding a hydroxyphenylpyruvate reductase, and its expression in cell suspension cultures of Salvia officinalis. Plant Cell Tiss Organ Cult 114: 131-138.
- Spitzer-Rimon, B., Cna’ani, A. and Vainstein, A. (2013) Virus-aided gene expression and silencing using TRV for functional analysis of floral scent-related genes. Methods in Molecular Biology 975: 139-148.
- Farhi, M., Kozin, M., Duchin, S. and Vainstein, A. (2013) Metabolic engineering of plants for artemisinin synthesis. Biotechnology & Genetic Engineering Reviews 29: 135-148.
- Spitzer, B., Farhi, M., Albo, B., Cna’ani, A., Moyal Ben Zvi, M., Masci, T., Edelbaum, O., Yu, Y., Shklarman, E., Ovadis, M. and A., Vainstein, A. (2012) The R2R3-MYB‚Äìlike regulatory factor EOBI, acting downstream of EOBII, regulates scent production by activating ODO1 and structural scent-related genes. Plant Cell 24: 5089-5105.
- Vainstein, A., Marton, I., Zipin Rotman, A., De Costa, N., Honig, A., Marhevka, E., Omid A. and Zuker A. (2012) Permanent genome modifications in plant cells by transient viral vectors. Acta Horticulturae 953: 31-36
- Moyal Ben Zvi, M., Shklarman, E., Masci, T., Kalev, H., Debener, T., Shafir, S., Ovadis, M. and Vainstein, A. (2012) PAP1 transcription factor enhances production of phenylpropanoid and terpenoid scent compounds in rose flowers. New Phytologist 195:335-345.
- Chernin, L., Toklikishvili, N., Dandurishvili, N., Tediashvili, M. and Vainstein, A. (2012) Suppression of crown gall disease by rhizosphere bacteria and Agrobacterium-specific bacteriophages. In ‚"Molecular Microbial Ecology of The Rhizosphere‚" (Frans J. de Bruijn, ed.), Wiley-Blackwell, v. 2, pp. 607-613.
- Alon, M., Elbaz, M., Moyal Ben-Zvi, M., Feldmesser, E., Vainstein, A. and Morin, S. (2012) Insights into the transcriptomics of polyphagy: Bemisia tabaci adaptability to phenylproapnoids involves coordinated expression of defense and metabolic genes. Insect Biochem. Mol. Biol. 42:251-263.
- Tzfira, T., Weinthal, T., Marton, I., Zeevi, V., Zuker, A. and Vainstein, A. (2012) Genome modifications in plant cells by custom-made restriction enzymes. Plant Biotech. Journal 10:373-389.
- Farhi, M., Marhevka, E., Ben-Ari, J., Algamas-Dimantov, A., Liang, Z., Zeevi, V., Edelbaum, O., Spitzer-Rimo, B., Abeliovich, H., Schwartz, B., Tzfira, T. and Vainstein, A. (2011) Generation of the potent anti-malarial drug artemisinin in tobacco. Nature Biotechnology 29:1072-1074.
- Chernin, L., Toklikishvili, N., Ovadis, M., Kim, S., Ben-Ari, J., Khmel, J. and Vainstein, A. (2011) Quorum-sensing quenching by rhizobacterial volatiles. Environmental Microbiology Reports 3:698-704.
- Farhi,M., Marhevka, E., Masci, T., Marcos, E., Eyal, Y., Ovadis, M., Abeliovich, H. and Vainstein, A. (2011) Harnessing yeast subcellular compartments for the production of plant terpenoids. Metabolic Eng.13:474-481.
- Vainstein, A., Marton, I., Zuker, A., Danziger, M and Tzfira, T. (2011) Permanent genome modifications in plant cells by transient viral vectors. Trends in Biotech. 26:363-369
- Zeng L., Wang Z., Vainstein A., Chen S. and Ma H. (2011) Cloning, localization and expression analysis of a new tonoplast monosaccharide transporter from Vitis vinifera L. J. Plant Growth Regul. 30:199-212.
- Krichevsky, A., Meyers, B., Vainstein, A., Maliga, P. and Citovsky, V. (2010) Autoluminescent Plants. PLoS ONE 5 (11): e15461. doi:10.1371/journal.pone.0015461
- Marton I., Zuker A., Shklarman E., Zeevi V., Tovkach A., Roffe S., Ovadis M., Tzfira T. and Vainstein A. (2010) Non-transgenic genome modification in plant cells. Plant Physiol. 154:1079-1087.
- Dandurishvili N., Toklikishvili N., Ovadis M., Eliashvili P., Giorgobiani N., Keshelava R., Tediashvili M., Vainstein A., Khmel I., Szegedi E. and Chernin L. (2010) Broad-range antagonistic rhizobacteria Pseudomonas fluorescens and Serratia plymuthica suppress Agrobacterium crown-gall tumors on tomato plants. J. Appl. Microbiol. 110:341-352.
- Toklikishvili, N., Dandurishvili, N., Vainstein, A., Tediashvili, M., Giorgobiani, N., Lurie, S., Szegedi, E., Glick, B.R. and Chernin, L. (2010) ACC deaminase-producing bacteria inhibit crown gall formation in tomato plants infected by Agrobacterium tumefaciens or A. vitis. Plant Pathology 59:1023-1030.
- Spitzer-Rimon, B., Marhevka, E., Barkai, O., Marton, I., Edelbaum, O., Masci, T., Prathapani, N., Shklarman, E., Ovadis, M. and Vainstein, A. (2010) EOBII, a gene encoding a flower-specific regulator of phenylpropanoid volatiles' biosynthesis in petunia. Plant Cell 22:1961-1976.
- Farhi, M., Lavie, O., Masci, T., Hendel-Rahmanim, K., Weiss, D., Abeliovich, H. and Vainstein, A. (2010) Identification of rose phenylacetaldehyde synthase by functional complementation in yeast. Plant Mol. Biol. 72:235-245.
- Moyal Ben Zvi, M., Zuker, A., Ovadis, M., Shklarman, E., Ben-Meir, H., Zenvirt, S. and Vainstein, A. (2008) Agrobacterium-mediated transformation of gypsophila (Gypsophila paniculata L.) Mol. Breeding 22:543-553.
- Dafny-Yelin, M., Tzfira, T., Vainstein, A. and Adam, Z. (2008) Non-redundant functions of sHSP-CIs in acquired thermotolerance and their role in early seed development in Arabidopsis. Plant Mol. Biol. 67:363-373.
- Moyal Ben Zvi , M., Negre-Zakharov, F., Masci, T., Ovadis, M., Shklarman, E., Ben-Meir, H., Tzfira, T., Dudareva, N., Vainstein, A. (2008) Interlinking showy traits: co-engineering of scent and color biosynthesis in flowers. Plant Biotech. Journal 8:403-415.
- Glick, A., Philosoph-Hadas, S., Vainstein, A., Meir, A., Tadmor Y., Meir S. (2007) Methyl jasmonate enhances color and carotenoid content of yellow-pigmented cut rose flowers. Acta Hort. 755:243-250.
- Hendel-Rahmanim, K., Masci, T., Vainstein, A., Weiss, D. (2007) Diurnal regulation of scent emission in rose flowers. Planta 226:1491-1499.
- Spitzer, B., Moyal Ben Zvi, M., Ovadis, M., Marhevka, E., Barkai, O., Edelbaum, O., Marton, I., Masci, T., Alon, M., Morin, S., Rogachev, I., Aharoni, A., Vainstein, A. (2007) Reverse genetics of floral scent: application of TRV-based gene silencing in petunia. Plant Physiol. 145:1241-1250.
- Ben-Zvi, M., Spitzer, B. and Vainstein, A. (2005) Secrets of floral scent. Flower World (Hebrew) 12:42-44.
- Citovsky, V., Lee, L-Y., Vyas, S., Glick, E., Chen, M-H., Vainstein, A., Gafni, Y., Gelvin, S.B., Tzfira, T. (2006) Subcellular localization of interacting proteins by bimolecular fluorescence complementation in planta. J Mol. Biol. 362:1120-1131.
- Leitner-Dagan, Y., Ovadis, M., Shklarman, E., Elad, Y., Rav David, D., Vainstein, A. (2006) Spatial and temporal regulation of plastid lipid-associated protein CHRC supports its dual role. Plant Physiol. 142:233-244.
- Kaminaga, Y., Schnepp, J., Peel, G., Kish., C, Ben-Nissan., G, Weiss, D., Orlova, I., Lavie, O., Rhodes, D., Wood, K., Porterfield, DM., Cooper, AJL., Pichersky, E., Vainstein, A. and Dudareva, N. (2006) Phenylacetaldehyde synthase from Petunia hybrida is a bifunctional enzyme that catalyzes the efficient coupling of phenylalanine decarboxylation to phenylethylamine oxidation. J. Biol. Chem. 281:23357-23366.
- Moyal Ben Zvi, M., Spitzer, B., and Vainstein, A. (2006) Navigating the network of floral scent production. Acta Hort. 714:143-154.
- Leitner-Dagan, Y., Ovadis, M., Zuker, A., Shklarman, E., Ohad, I., Tzfira, T., Vainstein, A. (2006) CHRD, a plant member of the evolutionarily conserved YjgF family, is involved in photosynthesis and chromoplastogenesis. Planta 225:89-102.
- Farhi, M., Dudareva, N., Masci, T., Weiss, D., Vainstein, A. and Abeliovich, H. (2006) Synthesis of the food flavoring methyl benzoate by genetically engineered Saccharomyces cerevisiae Journal of Biotech. 122:307-315.
- Guterman, I., Masci, T., Chen, X., Negre, F., Pichersky, E., Dudareva, N., Weiss, D. and Vainstein, A. (2006) Generation of phenylpropanoid pathway-derived volatiles in transgenic plants: rose alcohol acetyltransferase produces phenylethyl acetate and benzyl acetate in petunia flowers. Plant Mol. Biol. 60: 555-563.
- Li, J., Vaidya, M., White, C., Vainstein, A., Citovsky, V. and Tzfira, T. (2005) Involvement of KU80 in T-DNA integration in plant cells. Proc. Natl. Acad. Sci. USA 102:19231-19236.
- Zaffryar, S., Zimerman, B., Abu-Abied, M., Belausov, E., Lurya, G., Vainstein, A., Kamenestky, R. and Sadot, E. (2007) Developmental specific association of microtubules with amyloplasts in scale cells of Narcissus tazetta. Protoplasma 230:153-163.
- Lacroix, B., Tzfira, T., Vainstein, A. and Citovsky, V. (2006) A case of promiscuity: Agrobacterium‚Äôs endless search for partners. Trends Genet. 22:29-37.
- Ben Zvi, M. and Vainstein, A. (2007) Carnation. In "Biotechnology in Agriculture and Forestry, Transgenic Crops VI" (Pua E.C. and Davey, M.R., eds.) Springer-Verlag Berlin, v. 61, pp. 241-252.
- Dafny-Yelin, M., Guterman, I., Menda, N., Ovadis, M., Shalit, M., Pichersky, E., Zamir, D., Lewinsohn, E., Adam, Z., Weiss, D. and Vainstein, A. (2005) Flower proteome-changes in protein spectrum during the advanced stages of rose petal development. Planta 222:37-46.
- Tzfira, T., Tian, G.W., Lacroix, B., Vyas, S., Li, J., Leitner-Dagan, Y., Krichevsky, A., Taylor, T., Vainstein, A. and Citovsky, V. (2005) pSAT vectors: a modular series of plasmids for fluorescent protein tagging and expression of multiple genes in plants. Plant Mol. Biol. 58: 503-516.
- Vainstein, A., Lewinsohn, E. and Weiss, D. (2005) A genomics approach for identification of floral scent genes in rose. In "Biology of Floral Scent" (Dudareva, N. and Pichersky, E, eds.) CRC Press, Boca Raton, FL, pp. 91-102.
- Casanova, E., Trillas, M.I., Moysset, L. and Vainstein, A. (2005) Influence of rol genes in floriculture. Biotech. Adv. 23:3-39.
- Shalit, M., Shafir, S., Larkov, O., Bar, E., Kaslassi, D., Adam, Z., Zamir, D., Vainstein, A., Weiss, D., Ravid, U. and Lewinsohn, E. (2004) Volatile compounds emitted by rose cultivars:fragrance perception by man and honey bees. Isr. J. Plant Sci. 52:245-255.
- Casanova, E, Valdés,, A.E., Zuker, A., Fernández, B., Vainstein, A., Trillas, M.I., Moysset, L. (2004) rolC-transgenic carnation plants: adventitious organogenesis and levels of endogenous auxin and cytokinins. Plant Sci. 167:551-560.
- Vainstein, A., Lewinsohn, E., Adam, Z., Pichersky, E., Zamir, D. and Weiss, D. (2003) Rose fragrance: genomic approaches and metabolic engineering. Acta Hort. (Forkmann, G., Hauser, B. and Michaelis, S., eds.) 612:105-111.
- Vainstein, A., Zamir, D. and Weiss, D. (2003) Rose fragrance--from gene to function. In "Encyclopedia of Rose Science", (Roberts, A., etc., eds.) Academic Press, Elsevier Science London, UK, pp. 263-265.
- Scovel, G. and Vainstein, A. (2003) Flowering in carnation and ways to manipulate flower characteristics. Flowering Newsletter 35: 34-40.
- Shalit, M., Guterman, I., Volpin, H., Bar, E., Tamari, T., Menda, N., Adam, Z., Zamir, D., Vainstein, A., Weiss, D, Pichersky, E. and Lewinsohn E. (2003) Volatile ester formation in roses: identification of an Acetyl-CoA:geraniol/citronellol acetyltransferase in developing rose petals. Plant Physiol. 131: 1868-1876.
- Casanova, E., Zuker, A., Trillas, M., Moysset, L. and Vainstein, A. (2003) The rolC gene in carnation exhibits cytokinin- and auxin-like activities. Scientia Horticulturae 97: 321-331.
- Guterman, I., Shalit, M., Menda, N., Piestun, D., Dafny-Yelin, M., Shalev, G., Davydov, O., Ovadis, M., Emanuel, M., Wang, J., Adam, Z., Pichersky, E., Lewinsohn, E., Zamir, D., Vainstein, A. and Weiss, D. (2002) Rose scent: genomic approach to discover novel floral fragrance-related genes. Plant Cell 14: 2325-2338.
- Lavid, N., Wang, J., Shalit, M., Guterman, I., Bar, E., Beuerle, T., Menda, N., Shafir, S., Zamir, D., Adam, Z., Vainstein, A., Weiss, D., Pichersky, E. and Lewinsohn, E. (2002) O-Methyltransferases involved in the biosynthesis of volatile phenolic derivatives in rose petals. Plant Physiol. 129: 1899-1907.
- Lavy, M., Zuker, A., Lewinsohn, E., Larkov, O., Ravid, U., Vainstein, A. and Weiss, D. (2002) Linalool and linalool oxide production in transgenic carnation flowers expressing the Clarkia breweri linalool synthase gene. Mol. Breeding 9: 103-111.
- Vainstein, A. (ed.) (2002) Breeding for ornamentals: classical and molecular approaches Kluwer Academic Publishers, 392 pp., Dordrecht, the Netherlands
- Ben-Meir, H., Zuker, A., Weiss, D. and Vainstein, A. (2002) Molecular control of floral pigmentation: anthocyanins. In "Breeding for ornamentals: classical and molecular approaches" (Vainstein, A. , ed.) Kluwer Academic Press, the Netherlands, pp. 253-272.
- Zuker, A., Tzfira, T., Ben-Meir, H., Ovadis, M., Shklarman, E., Itzhaki, H., Forkmann, G., Martens, S., Neta-Sharir, I., Weiss, D. and Vainstein, A. (2002) Modification of flower color and fragrance by antisense suppression of the flavanone 3-hydroxylase gene. Mol. Breeding 9:33-41.
- Guterman, I., Dafny-Yelin, M., Shalit, M., Emanuel, M., Shaham, N., Piestun, D., Zuker, A., Ovadis, M., Lavi, M., Lavid, N., Lewinsohn E., Pichersky, E., Vainstein, A. , Zamir, D., Adam, Z. and Weiss, D. (2001) An integrated genomic approach to discovering fragrance-related genes in rose petals. Flowering Newsletter 32:31-37.
- Scovel, G., Ovadis, M., Reuven, M., Ben-Yephet, Y. and Vainstein, A. (2001) Marker assisted selection for resistance to fusarium oxysporum in the greenhouse carnation. Acta Hort. (Van Huylenbroeck J., Van Bockstaele, E. and Debergh P., eds) 552:151-156.
- Vainstein, A. , Lewinsohn, E., Pichersky, E. and Weiss, D. (2001) Floral fragrance - new inroads into an old commodity. Plant Physiol. 127:1383-1389.
- Zuker, A., Shklarman, E., Scovel, G., Ben-Meir, H., Ovadis, M., Neta-Sharir, I., Ben-Yephet, Y., Weiss, D., Watad A. and Vainstein, A. (2001) Genetic engineering of agronomic and ornamental traits in carnation. Acta Hort. (Sorvari, S., Karhu, S., Kanervo, E. and Pihakaski, S., eds.) 560:91-94.
- Zuker A., Tzfira T., Scovel G., Ovadis A., Shklarman E., Itzhaki h. and Vainstein A. (2001) RolC-transgenic carnation with improved agronomic traits: quantitative and qualitative analyses of greenhouse-grown plants. J. Am. Soc. Hort. Sci. 126:13-18.
- Zuker, A., Tzfira, T., Ahroni, A., Shklarman, E., Ovadis, M., Itzhaki, H., Ben-Meir, H. and Vainstein, A. (2001) Genetic engineering of carnation (Dianthus caryophyllus). In "Biotechnology in Agriculture and Forestry" (Bajaj, Y.P.S., ed.) Springer-Verlag, Berlin, 48:70-83.
- Scovel G., Altshuler T., Liu Z. and Vainstein A. (2000) The evergreen gene is essential for flower initiation in carnation. J. Heredity 91:487-491.
- Ovadis, M., Zuker, A., Ahroni, A., Tzfira, T., Itzhaki, H., Shklarman, E., Ben-Meir, H. and Vainstein, A. (2000) Highly efficient procedure for generating transgenic carnation with novel traits. Acta Hort. (Le Nard, M. and Cadic,A., eds.) 508:49-51.
- Altman, A., Tzfira, T., Wang, W., Vinocur, B., Hazan, G. and Vainstein, A. (2000) Clonal stability, seasonal periodicity and transgenes: the lesson from long-term micropropagation of transgenic and non-transformed Populus tremula L. plants. Acta Hort. 530: 429-436.
- Vishnevetsky, M., Ovadis, M., Zuker, A. and Vainstein, A. (1999) Molecular mechanisms underlying carotenogenesis in the chromoplast: multilevel regulation of carotenoid-associated genes. Plant J. 20:423-431.
- Ovadis, M., Zuker, A., Ahroni, A., Tzfira, T., Itzhaki, H., Shklarman, E., Ben-Meir, H. and Vainstein, A. (1999) Application of an integrative system based on microprojectile bombardment and Agrobacterium tumefaciens to generate transgenic carnation plants with novel characteristics. In "Plant Biotechnology and In Vitro Biology" (Altman, A., Izhar, S. and Ziv, M., eds.) Kluwer Academic Press, the Netherlands, pp. 533-536.
- Zuker, A., Tzfira, T., Scovel, G., Shklarman, E., Ovadis, M., Itzhaki, H., Ben-Meir, H. and Vainstein, A. (1999) Transgenic carnation plants. Focus 5-6.
- Tzfira, T., Vainstein, A. and Altman, A. (1999) Rol-gene expression in transgenic aspen (Populus tremula) plants results in accelerated growth and improved stem production index. Trees 14:49-54.
- Ovadis, M., Chernin, L., Tzfira, T., Canaan, V., Aharoni, A., Sakar, D., and Vainstein, A. (1999) Transformation of tobacco and aspen plants with the ita locus of an INCQ plasmid confers resistance toAgrobacterium tumefaciens infection. In "Plant Biotechnology and In Vitro Biology" (Altman, A., Izhar, S. and Ziv, M., eds.) Kluwer Academic Press, the Netherlands, pp.189-192.
- Zuker, A., Ahroni, A., Tzfira, T., Ben-Meir, H. and Vainstein, A. (1999) Wounding by bombardment yields highly efficient Agrobacterium-mediated transformation of carnation (Dianthus caryophyllus L.). Mol. Breeding 5:367-375.
- Vishnevetsky, M., Ovadis, M. and Vainstein, A. (1999) Carotenoid sequestration in plants: the role of carotenoid-associated proteins. Trends Plant Sci. 4:232-235.
- Ovadis, M., Vishnevetsky, M. and Vainstein, A. (1998) Isolation and sequence analysis of a gene from Cucumis sativus (accession no. AF099501) encoding the carotenoid-associated protein CHRC. Plant Physiol. 118:1536.
- Tzfira, T., Vainstein, A. and Altman, A. (1998) Rol-transgenic Populus tremula: root development, root-borne bud regeneration and in vitro propagation coefficient. Trees 8:464-471.
- Scovel, G., Ben-Meir, H., Ovadis, M. and Vainstein, A. (1998) RAPD and RFLP markers tightly linked to the locus controlling carnation flower type. Theor. Appl. Genet. 96:117-122.
- Zuker, A., Tzfira, T. and Vainstein, A. (1998) Cut-flower improvement using genetic engineering. Biotech. Adv. 16:33-79.
- Tzfira, T., Jensen, C.S., Vainstein, A. and Altman, A. (1997) Agrobacterium tumefaciens-mediated transformation of Populus tremula L. through direct shoot regeneration from stem segments. Physiol. Plant. 99:554-561.
- Vishnevetsky, M., Ovadis, M., Itzhaki, H. and Vainstein, A. (1997) CHRC, encoding a chromoplast-specific carotenoid-associated protein, is an early gibberellic acid-responsive gene. J. Biol. Chem. 272:24747-24750.
- Ahroni, A., Zuker, A., Rozen, Y., Shejtman, H. and Vainstein, A. (1997) An efficient method for adventitious shoot regeneration from stem-segment explants of Gypsophila paniculata L. Plant Cell, Tissue Org. Cult. 49:101-106.
- Tzfira, T., Altman, A. and Vainstein, A. (1997) Transgenic Populus: a step-by-step protocol for its Agrobacterium-mediated transformation. Plant Mol. Biol. Rep. 15:219-235.
- Zuker, A., Ahroni, A., Shejtman, H. and Vainstein, A. (1997) Adventitious shoot regeneration from leaf explants of Gypsophila paniculata L. Plant Cell Rep. 16:775-778.
- Libal-Weksler, Y., Vishnevetsky, M., Ovadis, M. and Vainstein, A. (1997) Isolation and regulation of accumulation of a minor chromoplast-specific protein from Cucumis sativus corollas. Plant Physiol. 113:59-63.
- Vishnevetsky, M., Ovadis, M., Libal-Weksler, Y., Itzhaki, H. and Vainstein, A. (1997) Molecular analyses of carotenoid-associated proteins from chromoplasts of Cucumis sativus corollas. Acta Hort. (Altman, A. and Ziv, M., eds.) 447:575-578.
- Tzfira, T., Jensen, C.S., Vainstein, A. and Altman, A. (1997) Aspen transformation procedures: oncogenic Agrobacterium rhizogenes versus disarmed A. tumefaciens. Acta Hort. (Altman, A. and Ziv, M., eds.) 447:295-300.
- Jensen, C.S., Tzfira, T., Vainstein, A. and Altman, A. (1997) Direct regeneration and selection of Populus tremula L. transgenic shoots from. Agrobacterium tumefaciens-transformed stem explants. In "Biology of Root Formation and Development" (Altman, A. and Waisel, Y., eds.) Basic Life Sciences Series, Plenum Publishing Co., NY, pp. 209-211.
- Tzfira, T., Jensen, C.S., Vainstein, A. and Altman, A. (1997) Improved rooting ability and root-system performance in transgenic aspen plants. In "Biology of Root Formation and Development" (Altman, A. and Waisel, Y., eds.) Basic Life Sciences Series, Plenum Publishing Co., NY, pp. 181-186.
- Ben-Meir, H., Scovel, G., Ovadis, M. and Vainstein, A. (1997) Molecular markers in the breeding of ornamentals. Acta Hort. (Altman, A. and Ziv, M., eds.) 447:599-601.
- Zuker, A., Ahroni, A. and Vainstein, A. (1997) A highly efficient method for carnation transformation. Acta Hort. (Altman, A. and Ziv, M., eds.) 447:373-375.
- Vinocur, B., Tzfira, T., Ziv, M., Vainstein, A. and Altman, A. (1997) Bud regeneration and growth from transgenic and non-transgenic aspen (Populus tremula) root explants. In "Biology of Root Formation and Development" (Altman, A. and Waisel, Y., eds.) Basic Life Sciences Series, Plenum Publishing Co., NY, pp. 217-219.
- Altman, A., Pelah, D., Gal, A., Tzfira, T., Yarnitzky, O., Shoseyov, O. and Vainstein, A. (1996) Toward water stress-tolerant poplar and pine trees: molecular biology, transformation and regeneration. In "Somatic Cell Genetics and Molecular Genetics of Trees" (Ahuja, R. et al., eds.) Kluwer Academic Press, the Netherlands, pp. 47-56.
- Tzfira, T., Ben-Meir, H., Yarnitzky, O., Vainstein, A. and Altman, A. (1996) Highly efficient transformation and regeneration of transgenic aspen plants through shoot-bud formation in root culture, and transformation of Pinus halepensis. In "Somatic Cell Genetics and Molecular Genetics of Trees" (Ahuja, R. et al., eds.) Kluwer Academic Press, the Netherlands, pp. 125-130.
- Zuker, A., Ahroni, A., Chang, P.L., Cheah, K., Kononowicz, K.A., Woodson, W.R., Bressan, R.A., Watad, A.A., Hasegawa, P.M. and Vainstein, A. (1996) Transformation of carnation using the biolistic method. Plant Tissue Cult. Biotech. 2:105-108.
- Vishnevetsky, M., Ovadis, M., Itzhaki, H., Levy, M., Libal-Weksler, Y., Adam, Z. and Vainstein, A. (1996) Molecular cloning of a carotenoid-associated protein from Cucumis sativus corollas: homologous genes involved in carotenoid sequestration in chromoplasts. Plant J. 10:1111-1118.
- Tzfira, T., Vainstein, A. and Altman, A. (1996) Agrobacterium rhizogenes-mediated DNA transfer in Pinus halepensis. Plant Cell Rep. 16:26-31.
- Watad, A., Ahroni, A., Zuker, A., Shejtman, H., Nissim, A. and Vainstein, A. (1996) Adventitious shoot formation from carnation stem segments: a comparison of different culture procedures. Sci. Hortic. 65:313-320.
- Tzfira, T., Ben-Meir, H., Vainstein, A. and Altman, A. (1996) Highly efficient transformation and regeneration of transgenic aspen plants through shoot-bud formation on root cultures. Plant Cell Rep. 15:566-571.
- Tzfira, T., Vainstein, A. and Altman, A. (1996) A novel transformation procedure for Populus tremula. Plant Tissue Cult. Biotech. 2:109-112.
- Brandis, A., Vainstein, A. and Goldschmidt, E.E. (1996) Distribution of chlorophyllase among components of chloroplast membranes in Citrus sinensis organs. Plant Phys. Biochem. 34:49-54.
- Zuker, A., Ahroni, A., Cheng, P., Chea, K., Woodson., W.R., Bressan, R.A.,Watad, A., Hasegawa, P.M. and Vainstein, A. (1995) Transformation of carnation by microprojectile bombardment. Sci. Hortic. 64:177-185.
- Vainstein, A. (1995) Ornamental plant improvement: classical and molecular approaches. Chronica Hortic. 35:8-9.
- Vainstein, A. , Ben-Meir, H., Zuker, A., Watad, A., Scovel, G., Ahroni, A. and Ovadis, M. (1995) Molecular markers and genetic transformation in the breeding of ornamentals. Acta Hort. (Vainstein, A. and Weiss, D., eds.) 420:65-67.
- Libal-Weksler, Y., Vishnevetsky, M., Ovadis, M., Itzhaki, H. and Vainstein, A. (1995) Flower-specific carotenoid accumulation in chromoplasts: molecular control of carotenoid-associated proteins. Acta Hort. (Vainstein, A. and Weiss, D., eds.) 420:32-34.
- Altman, A., Yaari, A., Pelah, D., Gal, A., Tzfira, T., Wang, W.X., Shoseyov, O., Vainstein, A. and Riov, J. (1995) In vitro organogenesis, transformation and expression of drought-related proteins in forest tree cultures. In "Current Issues in Plant Molecular and Cellular Biology" (Terzi, M. et al., eds.) Kluwer Academic Publishers, the Netherlands, pp. 87-94.
- Vainstein, A. and Weiss, D. (eds.) (1995) Ornamental plant improvement: classical and molecular approaches. Acta Hort. 420, 149 pp., Leiden, the Netherlands.
- Vainstein, A. and Ben-Meir, H. (1994) DNA fingerprint analysis of roses. J. Amer. Soc. Hort. Sci. 119:1099-1103.
- Ben-Meir, H. and Vainstein, A. (1994) Assessment of genetic relatedness in roses by DNA fingerprint analysis. Sci. Hortic. 58:115-121.
- Vainstein, A. , Halevy, A., Smirra, I. and Vishnevetsky, M. (1994) Chromoplast biogenesis in Cucumis sativus corollas: rapid effect of GA3 on the accumulation of a chromoplast-specific carotenoid-associated protein. Plant Physiol. 104:321-326.
- Vainstein, A. , Ben-Meir, H. and Zuker, A. (1993) DNA fingerprinting as a reliable tool for the identification and genetic analysis of ornamentals. In "Creating Genetic Variation in Ornamentals" (Sciva, T. and Mercuri, A., eds.) EUCARPIA, Italy, pp. 373-387.
- Vainstein, A. (1993) Recent technical and scientific developments for the identification of varieties and the definition of minimum distances. In "The Protection of New Plant Varieties" CIOPORA, Switzerland, pp. 11-21
- Vainstein, A. , Fisher, M. and Ziv, M. (1993) Applicability of reporter genes to carnation transformation: comparison between chloramphenicol acetyltransferase and b-glucuronidase. HortScience 28:1122-1124.
- Vainstein, A. and Sharon, R. (1993) Biogenesis of petunia and carnation corolla chloroplasts: changes in the abundance of nuclear and plastid-encoded photosynthesis-specific gene products during flower development. Physiol. Plant. 89:192-198.
- Smirra, I., Halevy, A. and Vainstein, A. (1993) Isolation and characterization of a chromoplast-specific carotenoid-associated protein from Cucumis sativus corollas. Plant Physiol. 102:491-496.
- Broido, S., Loyter, A. and Vainstein, A. (1993) Transient expression of photosynthetic genes in transfected albinoid protoplasts and correct processing of newly synthesized chloroplast-destined polypeptides. Physiol. Plant. 88:259-266.
- Fisher, M., Ziv, M. and Vainstein, A. (1993) An efficient method for adventitious shoot regeneration from carnation petals. Sci. Hortic. 53:231-237.
- Vainstein, A. , Hillel, J., Lavi, U. and Tzuri, G. (1992) Genetic variation detected by DNA fingerprinting in flowers. Acta Hort. 314:345-351.
- Vainstein, A. , Fisher, M. and Ziv, M. (1992) Plant regeneration from carnation petals as a source for genetic variation in vitro. Acta Hort. 314:39-45.
- Sharon, D., Hillel, J., Vainstein, A. and Lavi, U. (1992) Application of DNA
fingerprinting for identification and genetic analysis of Carica papaya and other
Carica species. Euphytica 62:119-126.
and in CIOPORA News, Switzerland (1992).
- Vainstein, A. , Hillel, J., Lavi, U. and Tzuri, G. (1991) DNA fingerprints as a reliable tool for analysis of genetic relatedness in carnation. Euphytica 56:225-229
- Lavi, U., Hillel, J., Vainstein, A. and Sharon, D. (1991) Application of DNA fingerprints for the identification and genetic analysis of avocado. J. Amer. Soc. Hort. Sci. 116:1078-1081.
- Tzuri, G., Hillel, J., Lavi, U., Haberfeld, A. and Vainstein, A. (1991) DNA fingerprint analysis of ornamental plants. Plant Science 76:91-97.
- Broido, S., Loyter, A. and Vainstein, A. (1991) Expression of plant genes in transfected mammalian cells: accumulation of recombinant preLHC IIb proteins within cytoplasmic inclusion bodies. Exp. Cell Res. 192:248-255.
- Weiss, D., Shomer-Ilan, A., Vainstein, A. and Halevy, A.H. (1990) Carbon fixation in corollas of Petunia hybrida. Possible coordination between photosynthetic systems under low CO2 flow. Physiol. Plant. 78:345-350.
- Anandan, S., Vainstein, A. and Thornber, J.P. (1989) Correlation of some published amino acid sequences for photosystem I polypeptides to a 17 kDa LHC I pigment-protein and to subunits III and IV of the core complex. FEBS Lett. 256:150-154.
- Vainstein, A. , Razin, A., Graessmann, A. and Loyter, A. (1989) Fusogenic reconstituted Sendai virus envelopes as a vehicle for introduction of DNA into viable mammalian cells. In "Methods in Enzymology" (Wu, R., Grossman, L. and Moldave, K., eds.) Academic Press, New York, vol: Recombinant DNA Methodology, pp. 633-652.
- Thornber, J.P., Peter, G.F., Chitnis, P.R. and Vainstein, A. (1989) Molecular and cellular biology of the major light-harvesting pigment-protein (LHC IIb) of higher plants. In "Photosynthesis: Molecular Biology and Bioenergetics" (Singhal, G.S. et al., eds.) Springer-Verlag-Narosa Publishing House, New Dehli, pp. 373-387.
- Vainstein, A. , Peterson, C.C. and Thornber, J.P. (1989) Light-harvesting pigment-proteins of photosystem I in maize. J. Biol. Chem. 264:4058-4063.
- Vainstein, A. , Ferreira, P., Peterson, C.C., Verbeke, J. and Thornber, J.P. (1989) Expression of the major light-harvesting chlorophyll a/b-protein and its insertion into thylakoids of mesophyll and bundle sheath chloroplasts of maize. Plant Physiol. 89:602-609.
- Thornber, J.P., Peter, G.F., Chitnis, P.R., Nechushtai, R. and Vainstein, A. (1988) Some aspects of the molecular and cellular biology of the light-harvesting complex of photosystem II. In "Light Energy Transduction in Photosynthesis" (Stevens Jr., S.E. and Bryant, D.A., eds.) American Society of Plant Physiologists, Rockville, MD., pp.137-154.
- Vainstein, A. , Hershkowitz, M., Israel, S., Rabin, S. and Loyter, A. (1984) A new method for reconstitution of highly fusogenic Sendai virus envelopes. Biochim. Biophys. Acta 773:181-188.
- Loyter, A., Vainstein, A. , Graessmann, M. and Graessmann, A. (1983) Fusion-mediated injection of SV40-DNA: Introduction of SV40-DNA into tissue culture cells by the use of DNA-loaded reconstituted Sendai virus envelopes. Exp. Cell Res. 143:415-425.
- Vainstein, A. , Razin, A., Graessman, A. and Loyter, A. (1983) Fusogenic reconstituted Sendai virus envelopes as a vehicle for introduction of DNA into viable mammalian cells. In "Methods in Enzymology" (Wu, R. and Moldave, K., eds.) Academic Press, New York, vol. 101, pp. 492-512.
- Vainstein, A. , Atidia, J. and Loyter, A. (1981) Reconstituted Sendai virus envelopes as a biological carrier for microinjection of proteins and DNA molecules. In "Liposomes in Study of Drug Activity and Immunocompetent Cell Function" (Panaf, A. and Nicolau, C., eds.) Academico Press, New York, pp. 95-108.
Carotenoid-associated proteins useful for high carotenoid accumulation and production in plants and other organisms.
Method for enhancing plant scent.
Plant viral expression vectors and use of same for generating genotypic variations in plant genomes.
Generating genotypic variations in plant genomes by direct gamete infection.
Engineering Saccharomyces Cerevisiae for the production of terpenoids: mitochondrial subcellular localization of terpene synthases.
Production of artemisinin in a non host plants: Engineering Nicotiana tabacum for biosynthesis of anti-malarial drug.
Generation of pigments in flowers of genetically modified gypsophila
Born 1957, Tbilisi, Georgia
1976 B.Sc., State University of Tbilisi, USSR
1984 Ph.D. The Hebrew University of Jerusalem, Israel
1986-1988 Postdoctorate, University of California at Los Angeles, USA
1988-1993 Lecturer Department of Horticulture, Faculty of Agriculture, HUJ
1994-1999 Senior Lecturer, Department of Horticulture, Faculty of Agriculture, HUJ
1997-1998 Sabbatical in Dr. J. Cohen's laboratory, USDA, Beltsville, MD, USA
2000-2005 Associate Professor, Department of Horticulture, Faculty of Agriculture, HUJ
2003-2004 Sabbatical in Dr. V. Citovsky’s laboratory, State University of New York, Stony Brook, NY, USA
2006-present Professor, Institute of Plant Sciences and Genetics in Agriculture, HUJ
1990-present Member of the Otto Warburg Center for Biotechnology in Agriculture, Faculty of Agriculture, HUJ
1993 Recipient of the Ellis Birk Fund Award
1993-1999 Incumbent of the Cyril Rosenbaum Senior Lectureship in Horticulture
1999 Recipient of the Agritech 99 and the Ministry of Agriculture Award for Excellence
2001-2002 Head of the Department of Horticulture, Faculty of Agriculture, HUJ
2001-2004 Head of the “Lehava” (Towards Higher Education at the HUJ) Program
2001-2005 Head of the Faculty of Agriculture Graduate Horticulture Program, HUJ
2002-2005 Head of the Hebrew University Graduate Biotechnology Program
2002-2005 Israeli national representative to COST
2003-2004 President of the Israeli Society of Plant Sciences
2005-2008 Head of The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, HUJ
2005-present Incumbent of the Wolfson Family Chair in Floriculture
2010 Recipient of the Kaye Innovation Awards
2011-2012 Head of the Faculty of Agriculture Graduate Plant Sciences Program, HUJ
Intro to molecular biology 71065; BSc
Seminar 71187/8, MSc
Basic methods in molecular biology 71024, BSc
Research workshop in horticulture 71950, MSc
Classical and molecular approaches in plant breeding 71021, BSc
Biological membranes, 71978, MSc
Current lab members
Former students and associates:
Ronit Sharon, Photosynthetic apparatus in corollas of petunia and carnation M.Sc.
Galil Tzuri, M.Sc. in collaboration with Prof. J. Hillel
Morly Fisher, M.Sc. in collaboration with Prof. M. Ziv
Iris Smirra, M.Sc. in collaboration with Prof. A. Halevy
Vered Canaan, Transgenic plants resistant to crown gall, MSc
Shamir Tznuert, Transformation system for Gypsophila, MSc
Michal Lavy, Fragrance in carnation, co-supervisor D. Weiss, MSc
Ofer Cohen, Regeneration and transformation in roses, MSc
Eyal Capua, Triterpene saponin synthesis in Gypsophila, MSc
Ran Amir, Identification and characterization of the locus ita from the plasmid RSF1010, MSc
Chanan Himelfarb, Adaptation to salinity, co-supervisor A. Lers, MSc
Sarit Neder, Use of tachyplesin I for improvement of rose vase life, co-supervisor N. Golob, MSc
Coby Buchsdorf, Delay of flowering in leaf crops, co-supervisor A. Samach, MSc
Efrat Izhaki, Rose genomics,. MSc
Shely Zafriar, Cytoskeleton changes in Narcissus during development, co-supervisor E. Sadot, MSc
Hanoch Glasner, The use of knotted1 to delay senescence of leaves and to prolong vase life of flowers, co-supervisor N. Ori. MSc
Orly Lavie, Identification and characterization of rose gene for phenylethyl alcohol production. MSc
Yosefa Bar Zvi, Transgenic tobacco with tachiplesin I, co-supervisor N. Golob. MSc
Ben Spitzer, Regulation of floral scent in petunia.MSc
Yael Brand, Modification of flower color and scent in Gypsophila. MSc
Hadas Price, Molecular breeding of petunia. MSc
Boaz Albo, Transcription Factor EOB1: A Key Component in a Network Regulating Production of Phenylpropanoid Scent Compounds in Petunia Flowers, MSc
Shimshon Broido, Ph.D. in collaboration with Prof. A. Loyter
Michael Vishnevetsky, Carotenoid-associated proteins, degree completed. PhD
Tzvi Tzfira, PhD. in collaboration with Prof. A. Altman
Amir Zuker, Transgenic carnation plants with novel traits, degree completed. PhD
Gidon Scovel, Genetic characterization of horticultural traits in carnation, degree completed. PhD
Hagit Ben-Meir, Characteization of the flavonoid biosynthetic pathway in Gypsophila, degree completed. PhD
Inna Gutterman, Rose genomics, co-supervisor D. Weiss. PhD
Yael Leitner, Tomato proteins involved in the sequestration of hydrophobic molecules PhD
Mery Yelin, Rose propteome, co-supervisor Z. Adam. PhD
Michal Ben-Zvi, Metabolic flow within and between pathways for floral scent and color. PhD
Alon Glick, Molecular markers for resistance to Botrytis in rose, co-supervisor S. Meir. PhD
Arjan Koot, M.Sc. visiting researcher, Dept. of Plant Breeding, University of Wageningen, the Netherlands
Malka Bazak, M.Sc. researcher
Dogan Sakar Ph.D visiting professor, Turkey
Eva Casanova, visiting Ph.D. student, Departament de Biologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain
Oren Barkai, Transcriptional activation of volatile benzenoids in flowers. researcher
Emanuel Kohen, visiting researcher, Lion, France
Xinlu Chen, Ph.D., postdoctoral fellow
Qiaochun Wang, Ph.D., postdoctoral fellow
Natalia Dandurishvili, visiting researcher, Khanchaveli Institute of Plant Protection, Tbilisi, Georgia.
Naveen P. Kumar PhD Visiting scientist, Directorate of Floricultural Research, IARI Pusa Campus, New Delhi-110012