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David Weiss David Weiss

Tel: +972 8 9489436

Fax: +972 8 9489960

E-mail: david.weiss@mail.huji.ac.il

 

 

 

 

 

Research Interest

Hormone signal transduction and responses (gibberellin and cytokinin)

The plant hormones gibberellin and cytokinin regulate various developmental processes throughout the life cycle of the plant. Our lab studies the mechanisms of gibberellin and cytokinin signaling in Arabidopsis and tomato. We also study the role of phytohormones in plant adaptation to drought.

 

Major projects

  • Gibberellin signal transduction in tomato.
  • O-GlcNAc modification in hormone signaling.
  • Class I TCP transcription factors in hormone responses.
  • Gibberellin and DELLA proteins in plant response to abiotic stresses.

Teaching activities

Undergraduate courses

  • Plant Physiology - 71015
  • Physiology of flowering - 71449

Graduate courses

  • Biology and production of ornamental - 71155
  • Signal transduction and gene regulation in plants - 71101

Selected Publications

  • Farber M., Attia Z. and Weiss D. (2016). Cytokinin activity increases stomatal density and transpiration rate in tomato. J. Exp. Bot. (in press).
  • Steiner E., Livne S., Kobinson-Katz T., Mosquna A., Tarkowská D., Muller B., Tarkowski P. and Weiss D. (2016) SPINDLY inhibit class Ι TCP proteolysis to promote sensitivity to cytokinin. Plant Physiol. 171, 1485-1494.
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  • Livne S., Lor V.S., Nir I., Eliaz N., Aharoni A., Olszewski N.E., Eshed Y. and Weiss D. (2015) Uncovering DELLA-independent GA responses by characterizing new tomato procera mutants. Plant Cell 27: 1579-1594.
  • Manela N., Oliva M., Ovadia R., Sikron-Persi N., Biruk A., Fait A., Galili G., Perl A., Weiss D. and Oren-Shamir M. (2015) Phenylalanine and tyrosine levels are rate-limiting factors in production of health promoting metabolites in Vitis vinifera cv. Gamay Red cell suspension. Front Plant Sci. 16 July 2015
  • Nir I. Moshelion M. and Weiss D. (2014) The Arabidopsis GIBBERELLIN METHYL TRANSFERASE 1 suppresses gibberellin activity, reduces whole-plant transpiration and promotes drought tolerance in transgenic tomato. Plant Cell Environ. Plant Cell Environ. 37: 113–123
  • Rubinovich L., Ruthstein S. and Weiss D. (2014) The Arabidopsis cysteine-rich GASA5 is a redox-active metalloprotein that suppresses gibberellin responses. Mol. Plant 7: 244-247
  • Lor V.S., Starker C.G., Voytas D.F., Weiss D. and Neil E. Olszewski (2014). Targeted mutagenesis of the tomato PROCERA gene using TALENs. Plant Physiol. 166: 1288-1291.
  • Livne S and Weiss D. (2014) Cytosolic Activity of the Gibberellin Receptor GIBBERELLIN INSENSITIVE DWARF1A. Plant & Cell Physiol. 55: 1727-1733.
  • Boden S.A.*, Weiss D.*, Ross J.J., Davies N., Trevaskis B., Chandler P.M. and Swain S.M. (2014) EARLY FLOWERING3 regulates flowering in spring barley by mediating gibberellin production and FLOWERING LOCUS T expression. Plant Cell 26: 1557–1569. * Equal contribution
  • Steiner E., Yanai O., Efroni I., Ori N., Eshed Y. and Weiss D. (2012) Class I TCPs modulate cytokinin-induced branching and meristematic activity in tomato. Plant Signal. Behav. 7: 807-810
  • Steiner E., Efroni I., Gopalraj M., Saathoff K., Tseng T-S., Kieffer M., Eshed Y., Olszewski N. and Weiss D. (2012) The Arabidopsis O-GlcNAc transferase SPINDLY interacts with class I TCPs to facilitate cytokinin responses in leaves and flowers. The Plant Cell 24: 96–108
  • Fleishon S., Shani E., Ori N. and Weiss D. (2011) Negative reciprocal interactions between gibberellin and cytokinin in tomato. New Phytol. 190: 609-617
  • Rubinovich L. and Weiss D. (2010) The Arabidopsis cysteine-rich protein GASA4 promotes GA responses and exhibits redox activity in bacteria and in planta. Plant J. 64: 1018-1027
  • Shani E., Melnik H., Shleizer-Burko S., Burko Y., Weiss D. and Ori N. (2010) Cytokinin regulates compound leaf development. The Plant Cell 22:3206-3217
  • Bar-Akiva A., Ovadia R., Rogachev I., Bar-Or C., Bar E., Freiman Z., Nissim-Levi A., Gollop N., Lewinsohn E., Aharoni A., Weiss D., Koltai H. and Oren-Shamir M. (2010) Metabolic networking in Brunfelsia calycina petals after flower opening. J. Exp. Bot. 61: 1393 - 1403
  • Farhi M., Lavie O., Masci T., Hendel-Rahmanim K., Weiss D., Abeliovich H. and Vainstein A. (2010) Identification of rose phenylacetaldehyde synthase by functional complementation in yeast. Plant Mol. Biol. 72: 235-245
  • Maymon I., Greenboim-Wainberg Y., Sagiv S., Kieber J.J., Moshelion M., Olszewski N. and Weiss D. (2009) Cytosolic activity of SPINDLY implies the existence of a DELLA-independent gibberellin-response pathway. Plant J. 58: 979-988
  • Hendel-Rahmanim K., Masci T., Vainstein A. and Weiss D. (2007) Diurnal regulation of scent emission in rose flowers. Planta, 226: 1491-1499.
  • Weiss D. and Ori N. (2007) Mechanisms of cross-talk between gibberellin and other hormones. Plant Physiol. 144: 1240-1246
  • Varbanova M., Yamaguchi S., Yang Y., McKelvey K., Hanada A., Borochov R., Yu F., Jikumaru Y., Ross J., Cortes D., Ma C-J., Noel J., Mander L., Shulaev V., Kamiya Y., Rodermel S., Weiss D. and Pichersky E. (2007) Methylation of gibberellins by Arabidopsis GAMT1 and GAMT2. The Plant Cell 19: 32-45.
  • Wigoda N., Ben-Nissan G., Granot D., Schwartz A. and Weiss D. (2006) The gibberellin-induced, cysteine-rich protein GIP2 from Petunia hybrida exhibits in-planta antioxidant activity. Plant J. 48: 796-805.
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  • Kaminaga Y., Schnepp J., Peel G., Kish C.M., Ben-Nissan G., Weiss D., Orlova I., Lavie O., Rhodes D., Wood K., Porterfield M., Cooper A.J.L., Schloss J.V., Pichersky E., Vainstein A., and Dudareva N. (2006) Plant phenylacetaldehyde synthase is a bifunctional homotetrameric enzyme that catalyzes phenylalanine decarboxylation and oxidation. J. Biol. Chem. 281: 23357-23366.
  • Farhi M., Dudareva N., Masci T., Weiss D., Vainstein A. and Abeliovich H. (2006) Synthesis of the food flavoring methyl benzoate by genetically engineered Saccharomyces cerevisiae. J. Biotech. 122: 307-315.
  • Guterman I., Masci T., Chen X., Negre F., Pichersky E., Dudareva N., Weiss D. and Vainstein A. (2006) Generation of phenylpropanoid pathway-derived volatiles in transgenic plants: rose alcohol acetyltransferase produces phenylethyl acetate and benzyl acetate in petunia flowers. Plant Mol. Biol. 60: 555-563.
  • Dafny-Yelin M., Guterman E., Menda N., Ovadis M., Shalit M., Pichersky E., Zamir D., Lewinsohn E., Adam Z., Weiss D. and Vainstein A. (2005) Flower proteome - changes in protein spectrum during the advanced stages of rose petal development. Planta 222: 37-46.
  • Vaknin H., Bar-Akiva A., Ovadia R., Nissim-Levi A., Forer I., Weiss D., Oren-Shamir M. (2005) Active anthocyanin degradation in Brunfelsia calycina (Yesterday-Today-Tomorrow) flowers. Planta 222: 19-26.
  • Neta-Sharir I., Isaacson T., Lurie S. and Weiss D. (2005) Dual role for tomato HSP21: protecting photosystem II from oxidative stress and promoting color changes during fruit maturation. The Plant Cell 17: 1829-1838.
  • Katz E., Riov J., Weiss D. and Goldschmidt E. (2005) The climacteric-like behavior of young, mature and wounded citrus leaves. J. Exp. Bot. 56:1359-1367.
  • Greenboim-Wainberg Y., Maymon I., Borochov R., Alvarez J., Olszewski N., Ori N., Eshed Y. and Weiss D. (2005) Cross talk between gibberellin and cytokinin: the Arabidopsis GA-response inhibitor SPINDLY plays a positive role in cytokinin signaling. The Plant Cell 17: 92-102.
  • Katz E., Lagunes P., Riov J., Weiss D. and Goldschmidt E. (2004) Molecular and physiological evidence shows the existence of system I and system II ethylene production in Citrus fruit, a non-climacteric fruit. Planta 219: 243-252.
  • Ben-Nissan G., Lee J-Y., Borohov A. and Weiss D. (2004) GIP, a Petunia hybrida GA-induced cysteine-rich protein, has a role in shoot elongation and transition to flowering. The Plant J. 37: 229-238.
  • 2Shalit M., Guterman I., Volpin H., Bar E., Tamari T., Menda N., Adam Z., Zamir D., Vainstein A., Weiss D, Pichersky E. and Lewinsohn E. (2003) Volatile ester formation in roses: identification of an Acetyl-CoA :geraniol acetyltransferase in developing rose petals. Plant Physiol. 131: 1868-1876.
  • Li C-Y., Weiss D., and Goldschmidt E. (2003) Effects of carbohydrate starvation on gene expression in citrus root. Planta 217: 11-20.
  • Guterman I., Shalit M., Menda N., Piestun D., Dafny-Yelin M., Shalev G., Davydov O., Ovadis M., Emanuel M., Wang J., Adam Z., Pichersky E., Lewinsohn E., Zamir D., Vainstein A., and Weiss D. (2002) Rose scent: genomic approach to discovering novel floral fragrance-related genes. The Plant Cell 14: 2325-2338.
  • Lavid N., Wang J., Shalit M., Gutterman I., Bar E., Beuerle T., Menda N., Shafir S., Zamir D., Adam Z., Vainstein A., Weiss D., Pichersky E. and Lewinsohn E. (2002) O-methyltransferases involved in the biosynthesis of volatile phenolic derivatives in rose petals. Plant Physiol. 129: 1899-1907.
  • Lavy M., Zuker A., Larkov O., Ravid U., Lewinsohn E., Vainstein A. and Weiss D. (2002) Linalool and linalool oxid production in transgenic carnation flowers expressing the Clarkia breweri linalool-synthase gene. Mol. Breed. 9: 103-111.
  • Izhaki A., Borohov A., Zamski E. and Weiss D. (2002) Gibberellin regulates post-microsporogenesis processes in petunia anthers. Physiol. Plant. 115: 442-447.
  • Zuker A., Tzfira T., Ben-Meir H., Ovadis M., Shklarman E., Itzhaki H., Forkmann G., Martens S., Neta-Sharir I., Weiss D. and Vainstein A. (2002) Suppression of anthocyanin synthesis by antisense fht enhances flower fragrance. Mol. Breed. 9: 33-41.
  • Vainstein A., Lewinsohn E., Pichersky E. and Weiss D. (2001) Floral fragranceׁnew inroads into an old commodity. Plant Physiol. 127: 1383-1389.
  • Izhaki A., Swain S.M., Tseng T., Borochov A., Olszewski N.E. and Weiss D. (2001) The role of SPY and SPY's TPR domains in the regulation of gibberellin action throughout the life cycle of Petunia hybrida plants. The Plant J. 28: 181-190.
  • Weiss D. (2000) Regulation of flower pigmentation and growth: multiple signaling pathways control anthocyanin synthesis in expanding petals. Physiol. Plant. 110: 152-157.
  • Neta-Sharir I., Shoseyov O. and Weiss D. (2000) Sugar enhance the expression of GA-induced genes in developing petunia flowers: a possible role for hexokinase in sugar-sensing. Physiol. Plant. 109: 196-202.
  • Leitner-Dagan Y. and Weiss D. (1999) Ca2+, calmodulin and protein dephosphorylation are required for GA-induced gene expression in petunia corolla. Physiol. Plant. 105: 116-121.
  • Sabehat A., Weiss D. and Lurie S. (1998) Heat-shock proteins and cross-tolerance in plants. Physiol. Plant. 103: 437-441.
  • Sabehat A., Lurie S. and Weiss D. (1998) Expression of small heat-shock proteins at low temperatures: a possible role in protecting against chilling injuries. Plant Physiol. 117: 651-658.
  • Sabehat A., Lurie S. and Weiss D. (1998) Isolation and characterization of a heat-induced gene, hcit2, encoding a novel 16.5 kDa protein: expression coincides with heat-induced tolerance to chilling stress. Plant Mol. Biol. 36: 935-939.
  • 3Moalem-Beno D., Tamari G., Dagan-Lightner Y., Borochov A. and Weiss D. (1996) Sugar dependent gibberellin-induced chalcone synthase gene expression in petunia corollas. Plant Physiol. 113, 419-424.
  • Ben-Nissan G. and Weiss D (1996) The petunia homologue of tomato gast1: transcript accumulation coincides with gibberellin-induced corolla cell elongation. Plant Mol. Biol. 32, 1067-1074.
  • Mol J.N.M., Jenkins G.I., Schafer E. and Weiss D. (1996) Signal perception, transduction and gene expression involved in anthocyanin biosynthesis. Crit. Rev. Plant Sci. 15, 525-557.
  • Moscovici S., Beno D. and Weiss D. (1996) Leaf-mediated light responses in petunia flowers. Plant. Physiol. 110, 1215-1222.
  • Sabehat A., Weiss D. and Lurie S. (1996) The correlation between heat shock protein accumulation and persistence and chilling tolerance in tomato fruit. Plant Physiol. 110, 531-537.
  • Tamari G., Borochov A. and Weiss D. (1995) Methyl jasmonate induces pigmentation and flavonoid gene expression in petunia corollas: a possible role in wound response. Physiol. Plant. 94, 45-50.
  • Weiss D., Luit van der A., Knegt E., Vermeer E., Mol J.N.M. and Kooter J.M. (1995) Identification of endogenous gibberellins in petunia flowers: Induction of anthocyanin biosynthetic gene expression and antagonistic effect of abscisic acid. Plant Physiol. 107, 695-702.

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Current Members of the Group

Evyatar Steiner
Evyatar Steiner
Research Associate

Ido Nir
Ido Nir
PhD student

Hagai Shohat
Hagai Shohat
MSc student

Natanella Eliaz
Natanella Eliaz
PhD student

Sivan Kubesi
Sivan Kubesi
MSc student

Iftah Marash
Iftah Marash
MSc student

Shula Blum
Shula Blum
technician

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