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Hanokh Czosnek Henryk Hanokh Czosnek

Tel: 972 8 9489249 (office), 972 8 9489903 (lab) 972 54 8820627 (mobile)

Fax: 972 8 9468265

E-mail: hanokh.czosnek@mail.huji.ac.il

Skype: czosnek1947

 

 

 

 

 

Research Interests

Interactions between viruses, their host plant and their insect vector

We are interested in deciphering these Interactions on the molecular level. Our virus of choice is the Tomato yellow leaf curl virus (TYLCV), a small ssDNA geminivirus, which infects tomato plants. TYLCV is transmit by an insect vector, the whitefly Bemisia tabaci. Using in situ localization, cell fractionation, gene silencing, microarray profiling and metabolome analyses, we are following the fate of TYLCV in the infected susceptible and resistant tomato plants, and in the insect vector. We are discovering the genes and gene networks underlying virus resistance, and the signaling cascades leading to infection and to resistance. We are studying the effect of TYLCV on the whitefly transcriptome, and applying the gene silencing technology to impair the insect development and its ability to transmit viruses.

Virus indexing and plant resistance in the Middle East

We are characterizing the viruses infecting vegetables (potato, tomato), cereals (wheat, barley, maize) and stone fruits (plum) in the Middle East and the Maghreb and selecting for resistant genotypes. We have developed tests based on ELISA, PCR and microarray platforms, allowing to identify and index these viruses. Breeding and selection programs are under way for certain crops to obtain resistant genotypes.

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Education

1971: M.Sc Institute of Molecular Biology, University Paris VII, France, under the supervision of Anne-Lise Haenni and Francois Chappeville.

1977: Teaching License in Chemistry, School of Education, Hebrew University of Jerusalem, Jerusalem, Israel ;

1978: Ph.D. Department of Biological Chemistry, Hebrew University of Jerusalem, Israel, under the supervision of Nathan de Groot and Abraham Hochberg.

1977-80: Post-doctorate, New York State Institute for Basic Research in Mental Disabilities, Staten Island, New York, U.S.A.

Teaching Activities 2011-14

Courses

Molecular genetics (undergraduate)
Genetic engineering
Selected Chapters in Plant Molecular Biology
Laboratory of Plant Molecular Biology and Genetic Engineering (graduate)
Introduction to Molecular Biology (undergraduate, Division for International Studies)

MSc Students

Shriki Oz, Fridman Lily, Popovski Smadar (with Ghanim M, ARO)

PhD Students:

Eybishtz Assaf, Kliot Adi (with Ghanim M, ARO), Levy David (with Lapidot M, ARO), Mahadav Assaf, Moshe Adi, Edelbaum-Sade Dagan, Zaigerman Haim (with Cohen H, HUJ)

Post doctorate fellows:

Hariton Aliza, Luan Jun-Bo, Perla Carlo

C.V.

Employment history

1996-: Professor, Department of Field and Vegetable Crops and Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
1989-96: Associate Professor, Department of Field and Vegetable Crops and Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
1985-88: Senior Lecturer, Department of Field and Vegetable Crops and Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
1980-84: Senior Research Scientist, Department of Cell Biology, The Weizmann Institute of Science, Rehovot, Israel.
1977-80: Senior Research Scientist (tenure position), New York State Institute for Basic Research in Mental Disabilities, Staten Island, New York, U.S.A.
1973-77: Assistant in Biochemistry, Department of Biological Chemistry, The Hebrew University of Jerusalem, Israel.
1971-72: Assistant in Biochemistry, Faculté de Médecine de Paris, France.

Positions held

Head of the genetics studies (1991-1994, 2000-2004, 2006-2009), Faculty of Agriculture.
Head, Department of Field Crops and Genetics (1998-99), Faculty of Agriculture.
Member of professional evaluation committees: Field Crops, Entomology, Biochemistry.
Member of the Senate of the Hebrew University of Jerusalem (1994-2002).

 

 

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Funded Research Projects (2005-2014)

  • 1. Management of diseases caused by whitefly-transmitted geminiviruses
    • 1.1. Development of tomatoes with combined resistance to Tomato yellow leaf curl virus (TYLCV) using both virus-derived resistance and molecular-assisted breeding.
      • With Vidavski S (Hebrew University of Jerusalem, Israel), Lapidot M (Volcani Center, Israel), Maxwell D and Nakhla M (University of Wisconsin at Madison, USA), Mazyad H and Shalaby M (Agricultural Research Center, Giza, Egypt), Iraqi N (Bethlehem University, Palestinian Authority), Anfoka G (Al-Balqa’ Applied University, Al-Salt, Jordan), Sedegui M (National Agronomic Research Institute, Rabat, Morocco).
      • Funded by the US AID-CDR Middle East Research and Cooperation Program (2003-2009).
    • 1.2. Development of tomato breeding lines with resistance to Ralstonia solanacearum and begomoviruses for Guatemala and Central America
      • With Mejia L (University of San Carlos, Guatemala City, Guatemala), Vidavski F (Tomatech Co, Israel), Lavi U and Tsror L (ARO, Israel), Scott JW (University of Florida, USA), Hanson P, Green S, Graham D and Wang JF (AVRDC, Taiwan), and Maxwell D (University of Wisconsin at Madison, USA).
      • Funded by the US AID CDR (2006-2010).
    • 1.3. Identification of the whitefly-transmitted geminivirus complexes affecting cassava production in the southern regions of Africa and development of cassava resistant lines.
      • With Rey C and Gray M (University of the Witwatersrand, Johannesburg, South Africa), N. Cossa (Agriculture Research Institute, Maputo, Mozambique) and Brown J (University of Arizona, Tucson, USA).
      • Funded by the US AID-CDR (2004-2011).
    • 1.4. Classical and molecular-assisted breeding of tomatoes resistant to Tomato curly stunt virus (ToCSV) and related viruses for small-scale farmers in South Africa and Mozambique
      • With Rey C (University of the Witwatersrand, Johannesburg, South Africa), Pietersen G (Agricultural Research center, Pretoria, South Africa), Mondjana A (Universidade Eduardo Mondlane, Maputo, Mozambique).
      • Funded by the US AID-CDR (2007-2012).
    • 1.5. Development of tomato with multi-resistances to Tomato yellow leaf curl virus, salinity, drought and heat.
      • With Lapidot M and Levine I (Israel), Mazyad M (Egypt), Anfoka G (Jordan), Iraqi N (Palestinian Authority), Fakhfakh H (Tunisia).
      • Funded by the US AID-CDR Middle East Research and Cooperation Program (2010-2015).
    • 1.6. The molecular basis of resistance to Tomato yellow leaf curl virus in tomato
      • With Schaller A (Germany), Anfoka G (Jordan) and Iraqi N (Palestinian Authority).
      • Funded by the DFG Tri-lateral Program (2012-2014).
    • 1.7. Deciphering the gene network sustaining tomato resistance to Tomato yellow leaf curl virus
      • Funded by the Israeli Ministry of Agriculture (2013-2015).
    • 1.8. A role for heat shock protein 70 in localization, transport and expression of Tomato yellow leaf curl virus in tomato plants and in the virus insect vector
      • Funded by the Israeli Science Foundation (2013-2015).
      • With Gorovits R (Faculty of Agriculture, Hebrew University of Jerusalem).
  • 2. Management of other plant viruses
    • 2.1. Microarray-based detection of plant viral and viral-like pathogens
      • With Barba M (Plant Pathology Research Institute, Rome, Italy), Hadidi A (USDA, MD, USA), Caglayan K (Mustafa Kemal University, Antakya, Turkey), Mazyad H (Agricultural Research Center, Giza, Egypt), Anfoka G (Al-Balqa' Applied University, Al-Salt, Jordan).
      • Funded by NATO Science for Peace Program (2006-2009).
    • 2.2. Monitoring of cereal virus and virus-like diseases for prevention through regional detection and quarantine systems
      • With Zeidan M (QRC-Al- Qasemi College, and Israeli Ministry of Agriculture), AboulAta EA (Plant Pathology Research Institute, ARC, Giza, Egypt), Anfoka G (Al-Balqa' Applied University, Al-Salt, Jordan).
      • Funded by the US AID-CDR Middle East Research and Cooperation Program (2008-2013).
  • 3. Functional genomics of the whitefly Bemisia tabaci
    • 3.1. Functional genomics characterization of the whitefly Bemisia tabaci begomovirus interaction: an EST and array-based transcript profiling approach.
      • With R. Shatters and C. McKenzie (USDA, ARS, USHRL, Fort Pierce, Florida, USA), and J. Brown (The University of Arizona, Tucson, USA)
      • Funded by the United States-Israel Binational Agricultural Research and Development Fund (BARD) (2003-2007).
    • 3.2. Identification of genes involved in circular transmission of begomoviruses in whiteflies
      • With H, Ghanim M (Israel), and Winter S (Germany)
      • Funded by The German Israeli Foundation for Research and Development (GIF) (2008- 2011)
    • 3.3. Dissection of whitefly-geminivirus interactions at the transcriptomic, proteomic and cellular levels
      • With Ghanim M (Israel), Cicero J, Brown J (USA)
      • Funded by The United States-Israel Binational Agricultural Research and Development Fund (BARD) (2008-2011)
    • 3.4. Comparative genomics of virus circulative transmission by two Homopteran insect pests to agriculture, the whitefly Bemisia tabaci and the green peach aphid Myzus persicae
      • With Ghanim M
      • Funded by the Israel Science Foundation (ISF) (2008-2010)
    • 3.5. Blocking begomovirus transmission to plants by silencing genes of their whitefly vector
      • With Liu S-S (Zhejiang University, Hangzhou, China)
      • Funded by the Israel Science Foundation (ISF) (2013-2016)

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