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PfFit
Nava Moran's Home Page
Menachem Moshelion's Home Page
PfFit
installer
You must make sure your screen is set to at least 1024x768
resolution to run PfFit.
PfFit is a three-part program enabling the calculation of osmotic
water
permeability, from the spherical, wall-less cell volume changes, evoked
by a change of osmolarity in the external medium. The Pf calculation
takes into account the rate of the osmolarity change. PfFit has been
written using MATLAB ver. 6.5, rel 13. , by Nava Moran and Menachem
Moshelion, with GUI written by Roy Novik, compiled and bundled for
distribution by Dvorah Weisman
Background
The basic assumptions and definitions are explained by Moshelion et
al., 2004, including the Supplemental Material on the web (DYNAMIC
CHANGES IN THE OSMOTIC WATER PERMEABILITY OF PROTOPLAST PLASMA
MEMBRANE, Menachem Moshelion*, Nava Moran* and Francois Chaumont, Plant
Physiol., 135 (2301-2317) [* Both authors contributed equally to this work].
IMPORTANT:
This program involves curve fitting. The ease of obtaining ÒresultsÓ
may be deceptive!
Check the reported values of the error, strive for making it as small
as possible, re-try the fit with many various sets of initial
guesses!!! Create simulated data, add "noise", then try to fit - to get
the feeling. Even a small improvement in error may be important!
Be cautious and skeptical!!
PROGRAM STRUCTURE
Invoking PfFit brings up a MATLAB window with a ÔSTARTÓ button;
pressing it opens the main panel with 3 choices, each invoking, in
turn, one of the 3 program parts:
A: ÒIndicatorFitÓ, for fitting the experimentally sampled time
course
of concentration changes of the bath indicator,
B: ÒModelMakeÓ, for creating simulations of volume changes, bath
osmoticum changes and Pf changes, and
C: ÒVolumeFitÓ, for fitting the experimentally determined time
course
of changes in cell volume.
ÒModelMakeÓ and ÒVolumeFitÓ use parameters describing the bath
concentration changes originating from ÒIndicatorFitÓ; ÒVolumeFitÓ and
ÒIndicatorFitÓcan be tested on simulations created by ÒModelMakeÓ.
Prof. Nava Moran
Rm. 2221, Bldg A
R.H. Smith Inst. Plant Sciences & Gen. Agric.
Fac. Agric., Food & Env. Qual. Sci.
The Hebrew University
Rehovot, 76100 Israel
ph. 972-8-9489324
fax:972-8-9489899
nava.moran@huji.ac.il
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